Nino Ushikishvili started playing piano at the age of four. A few years later she entered the Z. Paliashvili Tbilisi Central Music School for Gifted Children. In 1995 she graduated with honors from the V. Sarajishvili Tbilisi Conservatoire and in 1997 she completed postgraduate studies at the same conservatoire under the guidance of Professor Vanda Shiukashvili. 
In 1998, Nino received a full scholarship and continued her studies in the United States, where in 2004 she received her Artist Diploma at the Texas Christian University in Fort Worth (Texas, USA), and in 2005 Graduate Diploma with honors at the New England Conservatory of Music in Boston (Massachusetts, USA). Her teachers at various times were Tamás Ungár, Joseph Banowets, and Alexander Korsantia.
 
Nino Ushikishvili is a prize winner of various piano competitions, among them the World Piano Competition in Cincinnati, Ohio, the Nina Wideman International Piano Competition in Shreveport, Louisiana, and the Hilton Head International Piano Competition in Hilton Head, South Carolina. She has participated in masterclasses with world-renowned musicians such as Leon Fleisher, Christopher Elton, Boris Petrushansky, Mikhail Voskresensky, Benedetto Lupo and others.

While studying in the United States, Nino regularly gave concerts in various cities, notably  performances in Washington, New York, Dallas, Boston, and Los Angeles.

Nino Ushikishvili has many years of teaching experience. Currently, she is on the faculty of the Music Academy of the University of Georgia and is pursuing a Doctor of Musical Arts degree in Piano Performance at the Tbilisi State Conservatoire.